目的：检测不同时程海洛因依赖大鼠海马、杏仁核和额叶皮质discs 大同源物4（discs large ho-molog 4，DLG4）的蛋白表达，探讨海洛因依赖对突触后致密结构的影响。方法采用腹腔内递增注射海洛因的方法，建立海洛因依赖的大鼠模型，用免疫组织化学法检测海洛因依赖9、18和36 d大鼠海马、杏仁核和额叶皮质DLG4蛋白的表达，并与对照组进行比较。结果随海洛因依赖时间的延长，海马、杏仁核和额叶皮质DLG4蛋白的表达逐渐降低。结论海洛因依赖可影响海马、杏仁核和额叶皮质突触后致密结构，并随依赖时间的延长，影响更加明显。

Objective To observe the expression of discs large hom olog 4 (DLG4) protein in hippocam-pus, am ygdala and frontal cortex of rats and evaluate postsynaptic density in heroin dependence. Meth-ods The rat heroin dependent m odel was established by increasing intraperitoneal injection of heroin. DLG4 proteins in hippocam pus, am ygdala and frontal cortex of heroin dependent 9, 18, 36 days rats w ere detected with im munohistochem ical staining and com pared with that in the control group. Results DLG4 proteins in hippocam pus, am ygdala and frontal cortex w ere gradually reduced with extension of heroin dependent tim e. Conclusion Heroin dependence can affect postsynaptic density of hippocam pus, am ygdala and frontal cortex. The changes becom e m ore apparent with extension of heroin dependence tim e.